The correct option is B Lignin
Safranin reacts with lignin and stains it red. Therefore, only the lignified tissues such as xylem and sclerenchyma are turned red and all other tissues like parenchyma, collenchyma and phloem remain colourless. In plant tissues stained with this method, safranin O appears brilliant red in chromosomes, nuclei, lignified, suberized cell walls. Starch, protein and cutin can not be stained by safranin.
Thus, the correct answer is option B.