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Which enzyme ...

Question

Which enzyme has polymerizing activity in 5-3 direction but exonuclease activity in 3-5 direction and how

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Solution

DNA polymerase 1 has activities like polymerase, 3’ to 5’ exonuclease and 5’ to 3’ exonuclease.

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Q. Choose the correct answer from the alternatives given :
A DNA template plus primer with the structure
3 P - TG C GAATTAG C GACAT - P5
5 P - ATCGGTACGACGCTTAAC OH 3
(where P = a phosphate group) is placed in an in vitro DNA synthesis system containing

M g^{2 +}

, an excess of the four deoxyribonucleoside triphosphates, etc. and a mutant form of E. Coli DNA polymerase I that lacks 5

\to

3 exonuclease activity. The 5

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5 exonuclease activities of this aberrant enzyme are identical to those of normal E. Coli DNA polymerase I. It simply has no 5

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Q. Statement 1: PCR can be performed on a random unsequenced stretch of DNA.
Statement 2: Proofreading activity by Taq polymerase is done in 3’-5’ direction.

Q. Does DNA Polymerase also have exonuclease activity? Please explain.

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Q. Which one is an essential mineral, not constituent of any enzyme but stimulates the activity of many enzymes?

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BIOLOGY

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Standard XII Biology

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