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which enzyme is used to prevent the self ligation of vector DNA and toincrease the frequency of formation of recombinants

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Q. Which enzyme is required to prevent unwanted self-ligation of vector DNA molecules in recombinant DNA technology?

Q. To prevent recirculation of vector DNA and to increase the frequency of production of recombinant DNA

Q. Arrange the given steps involved in recombinant DNA technology in the correct order.

A. Amplification of desired DNA sequence (gene of interest) with PCR modification
B. Ligation of gene of interest with the vector
C. Removal of RNA and proteins to purify DNA
D. Insertion of recombinant DNA into the host
E. Treatment of cell with cell wall digesting enzyme followed by disruption of the cell membrane
F. Digestion of DNA of interest and vector with suitable restriction enzyme

Q. What is recombinant DNA technology called popularly ? Name the vectors and enzymes used in this technique.

Q. Arrange the following steps of formation of recombinant DNA in the correct sequence.
I. Complementary sticky ends are produced on the foreign DNA and the plasmid.
II. Foreign DNA and plasmids are mixed together
III. DNA ligase is used to seal the nicks in the sugar–phosphate backbone.
IV. Cut the foreign DNA (containing the DNA fragment of interest) and the plasmid with the same restriction enzyme.

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