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Question

Which helped in confirming that oxygen evolved in photosynthesis comes from water

A
H182O
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B
14CO2
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C
15NO3
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D
3H2O
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Solution

The correct option is A H182O

Cornelius van Niel in 1931, demonstrated that photosynthesis is a light-dependent reaction and proposed that oxygen that is evolved during photosynthesis originates from water.
Ruben and Kamen's photosynthesis experiment using heavy isotope of oxygen 18O in water, to yield 18O2 gas confirmed this hypothisis.

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