The correct option is C Reproductive isolation
Speciation is the formation of new species from the pre existing species.
It involves reproductive isolation in groups within the original population, the groups develop genetic variations leading to the formation of new species.
Types of reproductive isolation are as follows:
Temporal isolation - It is a type of prezygotic barrier where two populations are separated due to difference in mating time.
Behavioural isolation - It is prezygotic isolation in which there is no mate recognition.
Seasonal isolation - It is due to variations in breeding seasons.
Hence the correct option is
b. Reproductive isolation