Which is not necessary for obtaining protoplasts for somatic hybridisation?

Cellulose

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Primase

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Pectinase

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Both A and C

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Solution

The correct option is D Primase
Somatic hybridization is the in vitro fusion of isolated protoplasts to form a hybrid cell and its subsequent development to form a hybrid plant. For the enzymatic isolation, a safe method is carried out in a simple solution with a high osmotic pressure along with cell wall degrading enzymes like cellulase and pectinase. Primase is not required.

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