Question

Which method is suitable for transferring an alien DNA into a plant cell?

Answer 1

Alien DNA:

1. The DNA is transferred from unrelated species to Cultivated species, then DNA is referred to as alien DNA.
2. The biolistic method is suitable for transferring alien DNA into a plant cell.

Biolistic method:

1. The biolistic method (Gene gun method) is the direct introduction of substances of DNA or RNA into cells and tissues.
2. In this process, the cells are shot with the DNA substances.
3. The foreign DNA is coated with some heavy metal particles like gold or tungsten.
4. The coated particles are loaded in the gene gun and are made to release with high pressure in order to enter the target cell wall.
5. This in turn gives the foreign DNA to the penetrated cells.
6. This method falls under genetic engineering.
7. It is carried out in intact plant cells. It will lead to mutation or raising desired variety.