The correct option is D Substrate
The active site of an enzyme is a groove which accomodates the binding of the enzyme with the substrate and it happens by a catalytic reaction. An active site always accomodates the binding of a specific substrate which is suitable to bind with it. When the substrate binds to the active site of the enzyme, it forms enzyme-substrate comlex (ES complex) and it happens due to the catalytic actionSo, the correct option is (D).