Question

Which of the following about the Sanger’s chain termination method for DNA sequencing is true?

• A. Special radiolabelled dNTPs are used in this method.
• B. DNA is allowed to replicate multiple times.
• C. The dNTPs gets substituted randomly into the DNA while replication.
• D. Once the dNTPs get substituted, replication ends.

Answer 1

Answer: (A), (B), (C), (D)

The correct options are
A Special radiolabelled dNTPs are used in this method.
B DNA is allowed to replicate multiple times.
C The dNTPs gets substituted randomly into the DNA while replication.
D Once the dNTPs get substituted, replication ends.

Chain termination method of DNA Sequencing involves repeated replication of DNA fragment using radiolabelled dNTPs. These dNTPs can be taken up instead of the normal NTPs during the DNA replication. Insertion of a dNTP leads to the termination of the replication process. After several rounds of replication, you will find DNA fragments of all sizes. They can then be arranged and studied to know the exact sequence of the DNA fragment.