Which of the following act as a substrate for an enzyme that charges tRNA molecules?
I. Amino acid
II. ATP
III. tRNA
IV. snRNA

I only

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II only

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III only

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I, II, and III

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I, II, III, and IV

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Solution

The correct option is D I, II, and III
An aminoacyl-tRNA synthetase binds a specific amino acid, via a high-energy ester bond, to 2’ or 3’ hydroxyl of the terminal adenosine in the corresponding tRNA using the energy of ATP hydrolysis. This is then followed by the pairing of tRNA anticodon with a codon in the mRNA specifying the attached amino acid. So, ATP, tRNA, and amino acid are the substrate of aminoacyl-tRNA synthetase that charges the tRNA.
Thus, the correct answer is option D.

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