Which of the following are correct about type Ⅱ endonucleases?
(a) They require ATP for their activity.
(b) They are bifunctional enzymes having nuclease and methylase activity.
(c) They breaks the sugar-phosphate backbone at the recognition site.
(d) They are naturally found in prokaryotes.
Choose the correct option:
Which of the following are correct about type Ⅱ endonucleases?
(a) They require ATP for their activity.
(b) They are bifunctional enzymes having nuclease and methylase activity.
(c) They breaks the sugar-phosphate backbone at the recognition site.
(d) They are naturally found in prokaryotes.
Choose the correct option:
The correct option is D c and d
Endonucleases are of three types
Type I
Type II
Type III
Cut the DNA far from the recognition site
Cut the DNA at the recognition site
Cut the DNA 20-30 bp away from the recognition site
Requires ATP for its function
ATP is not required for its function
Requires ATP for its function
Bifunctional enzyme has both nuclease and methylase activity
Unifunctional enzyme has only nuclease activity
Bifunctional enzyme has both nuclease and methylase activity
Examples- EcoKⅠ
Examples- EcoRⅠ
Examples- EcoPⅠ
Restriction endonucleases are naturally present in the bacteria(prokaryotes) where they provide protection against invading bacteriophage DNA.
Endonucleases are of three types
|
Type I |
Type II |
Type III |
|
Cut the DNA far from the recognition site |
Cut the DNA at the recognition site |
Cut the DNA 20-30 bp away from the recognition site |
|
Requires ATP for its function |
ATP is not required for its function |
Requires ATP for its function |
|
Bifunctional enzyme has both nuclease and methylase activity |
Unifunctional enzyme has only nuclease activity |
Bifunctional enzyme has both nuclease and methylase activity |
|
Examples- EcoKⅠ |
Examples- EcoRⅠ |
Examples- EcoPⅠ |
Restriction endonucleases are naturally present in the bacteria(prokaryotes) where they provide protection against invading bacteriophage DNA.
