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Question

Which of the following are limitations of using plasmids as vectors?

A
Inability to uptake large insert size
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B
Inability to express eukaryotic genes efficiently
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C
Inability to express prokaryotic genes
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D
Ability to uptake small insert size
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Solution

The correct option is B Inability to express eukaryotic genes efficiently
Plasmids have some limitations when used as vectors. The insert size is very small, upto 6 or 7 kb. When eukaryotic genes are inserted into plasmids, it is not efficiently expressed. To overcome these limitations, scientists have come with many new vectors such as phages, cosmids, BAC and YAC.

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