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Question
Which of the following criteria must be considered while selecting enzyme for the ELISA?
Which of the following criteria must be considered while selecting enzyme for the ELISA?
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Solution
The correct option is D All of the above
The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. The secondary antibody is conjugated to the enzyme which can act on the chromogenic substrate. The enzyme should be pure to react on the substrate, sensitive to give accurate results and stable. If these qualities are not maintained properly, the results which are obtained will not be accurate and proper.Thus, the correct answer is option D.
The method of ELISA is used for the detection of the disease using the antigen and antibody interaction. The secondary antibody is conjugated to the enzyme which can act on the chromogenic substrate. The enzyme should be pure to react on the substrate, sensitive to give accurate results and stable. If these qualities are not maintained properly, the results which are obtained will not be accurate and proper.
Thus, the correct answer is option D.
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