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Question
Which of the following enzyme is used to join DNA fragments?
Which of the following enzyme is used to join DNA fragments?
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Solution
The correct option is D Ligase
DNA ligase joins the annealed cohesive ends produced by certain restriction enzymes and also catalyze the formation of phosphodiester bonds between blunt-ended fragments of DNA to join them together. DNA polymerase catalyzes the polymerization of nucleotides to form DNA and requires both a primer (a short oligonucleotide strand), to which nucleotides are added, and a template strand to guide selection of each new nucleotide. Endonucleases are the enzymes that breaks phosphodiester bond within a polynucleotide chain. For example, restriction endonucleases recognize and cleave DNA at specific DNA sequences (recognition sequences or restriction sites) to generate a set of smaller fragments. Terminase enzymes are involved in cutting and packaging the viral genome.So, the correct answer is 'Ligase'
DNA ligase joins the annealed cohesive ends produced by certain restriction enzymes and also catalyze the formation of phosphodiester bonds between blunt-ended fragments of DNA to join them together. DNA polymerase catalyzes the polymerization of nucleotides to form DNA and requires both a primer (a short oligonucleotide strand), to which nucleotides are added, and a template strand to guide selection of each new nucleotide. Endonucleases are the enzymes that breaks phosphodiester bond within a polynucleotide chain. For example, restriction endonucleases recognize and cleave DNA at specific DNA sequences (recognition sequences or restriction sites) to generate a set of smaller fragments. Terminase enzymes are involved in cutting and packaging the viral genome.
So, the correct answer is 'Ligase'
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