Question

Which of the following enzyme will get inactivated in insertional inactivation?

• A. Transacetylase
• B. Permease
• C. $\beta -$galactosidase
• D. Taq-polymerase

Answer 1

The correct option is C $\beta -$galactosidase

The insertion of $\text{rDNA}$ into the coding sequence of an enzyme $\beta -$galactosidase leads to the inactivation of the enzyme called insertional inactivation. The recombinants do not produce blue coloured colonies in the presence of chromogenic substrate while the non-recombinants produce a blue colour.

So, the correct answer is $\beta -$galactosidase.