Insulin in Humans

Q.

Name a protein that functions as a hormone.

Q. Which of the following enzyme will get inactivated in insertional inactivation?

1. Transacetylase
2. Permease
3. $\beta -$galactosidase
4. Taq-polymerase

Q. Mature Insulin does not contain -------

1. A peptide
2. B peptide
3. C peptide
4. Disulphide bonds

Q. ntArrange the following in increasing bond angle~n ntOF2 OCl2 OBr2 OI2n

Q. Give a schematic representation of the transformation of a pro-insulin to insulin. [2 Marks]

Q.

LH is secreted by

1. Hypothalamus

2. Pars intermedia

3. Thyroid

4. Pars distalis

Q. The processing of proinsulin into mature insulin includes

1. addition of C peptide
2. removal of C peptide
3. removal of A peptide
4. removal of B peptide

Q. Read the following statements and identify the correct statement.

1. In $1997,$ an American company got patent rights on Basmati rice through the International Patent and Trademark office
2. There are an estimated $50,000$ varieties of rice in India alone
3. First clinical gene therapy was given in $1994$ to $4-$year old girl with adenosine deaminase deficiency
4. In $1983,$ Eli Lily Company prepared $DNA$ sequences corresponding to $A,B$ and $C$ chains of human insulin

Q. C-peptide of human insulin is _________________.

1. Removed during maturation of pro-insulin to insulin
2. Responsible for its biological activity
3. A part of mature insulin molecule
4. Responsible for formation of disulphide bridges

Q. Can you list $10$ recombinant proteins which are used in medical practice? Find out where they are used as therapeutics.

Q. Match list I with list II and select the correct option
$\begin{array}{cc}ListI& ListII\\ \left(a\right)Bacillusthuringiensis& 1.Productionofchitinases\\ \left(b\right)Rhizobium& 2.Scavengingofoilspills\\ \left(c\right)Escherichiacoli& 3.Incorporationof{}^{\prime }ni{f}^{\prime }gene\\ \left(d\right)Pseudomonas& 4.productionofBttoxin\\ \left(e\right)Trichoderma& 5.Productionofhumaninsulin\end{array}$

1. a-2, b-4, c-1, d-5, e-3
2. a-4, b-3, c-5, d-2, e-1
3. a-2, b-4, c-5, a-1, e-3
4. a-3, b-4, c-5, d-1, e-2

Q.

The number of amino acids in insulin molecule:

1. 41

2. 51

3. 61

4. 71

Q. Mature insulin does not contain

1. A peptide
2. B peptide
3. C peptide
4. Disulphide bonds

Q. Human insulin contains how many amino acids on B chain?

1. $51$
2. $21$
3. $30$
4. $31$

Q. In Insulin molecule which bond joins chain A and Chain B together?

Q. __________ is a globular protein of $\sim 6kDa$ consisting of $51$ amino acids, arranged in $2$ polypeptide chains held together by disulphide bridge.

1. Insulin
2. Keratin
3. Glucagon
4. Fibrinogen

Q. During the processing of proinsulin into mature insulin

1. B-peptide is added to proinsulin
2. C-peptide is removed from proinsulin
3. C-peptide is added to proinsulin
4. B-peptide is removed from proinsulin.

Q.

Which of the following is true about human insulin?

1. Human insulin has two polypeptide chains.

2. Two polypeptide chains are connected by disulphide bridges.

3. Proinsulin has A, B and C chains.

4. All of the above.

Q. Describe briefly the structure of insulin. How is gentically engineered insulin synthesized ?

Q. Select the correct options to fill up the blanks.
(i) ______enzymes is crucial for the immune system to function and its absence is caused by the deletion of a gene.
(ii) Insulin consists of _____and _____that are linked together by ____.
(iii) Transgenic mice are being used to test the safety of the _____.
(iv)______involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA.

1. (i) Adenosine deaminase, (ii) A-chain, B-chain, disulfide bridges, (iii) polio vaccine, (iv) RNAi
2. (i) Adenosine deaminase, (ii) A-chain, B-chain, hydrogen bonds, (iii) polio vaccine, (iv) RNAi
3. (i) RNAi, (ii) A-chain, B-chain, non-covalent bridges, (iii) polio vaccine. (iv) adenosine deaminase
4. (i) RNAi, (ii) A-chain, B-chain, disulphide bridges, (iii) adenosine deaminase, (iv) polio vaccine

Q. Which of the following statements is not correct?

1. The proinsulin has an extra peptide called C-peptide
2. The functional insulin has A and B chains linked together by hydrogen bonds
3. Genetically engineered insulin is produced in E-coli
4. In man insulin is synthesised as a proinsulin

Q. The two polypeptides of human insulin are linked together by:

1. Hydrogen bonds
2. Phosphodiester bond
3. Disulphide bridges
4. Covalent bond

Q. Given figure represents the maturation of pro-insulin into insulin. Identify the product A.

1. Both A and B
2. Polypeptide chain A
3. Polypeptide chain B
4. Polypeptide chain C

Q. How many polypeptides are found in human insulin?

Q. The two polypeptides of human insulin linked together by

1. Covalent bond
2. Hydrogen bonds
3. Disulphide bridges
4. Phosphodiester bond

Q.

Which of the following is true about human insulin?

1. Human insulin has two polypeptide chains.

2. Two polypeptide chains are connected by disulphide bridges.

3. Proinsulin has A, B and C chains.

4. All of the above.

Q. The two polypeptides of human insulin are linked together by

1. Disulphide bridges
2. Hydrogen bonds
3. Phosphodiester bridge
4. Covalent bond

Q. Identify the wrong statement with reference to the structure of human insulin :

1. A and B chains are combined by disulphide bonds to form humulin.
2. Insulin is synthesised as a pre-hormone which contains an extra stretch called C peptide.
3. Human insulin is made up of 51 amino acids arranged in two polypeptide chains.
4. In these, chain A is made up of 30 amino acids and chain B is made up of 21 amino acids.

Q. Mature insulin is different from pro-insulin in

1. Having more number of nucleotides
2. The absence of C - Peptide
3. Having more number of amino acids
4. Causing allergic reactions

Q. Mature Insulin does not contain_____

1. A peptide
2. B peptide
3. C peptide
4. Disulphide bonds