Question

Which of the following enzymes are used as the tools of recombinant DNA technology?

• A. Restriction enzymes, polymerase enzymes, lipase
• B. Restriction enzymes, polymerase enzymes, ligases
• C. Restriction enzymes, lipase, ligases
• D. Restriction enzymes, polymerase enzymes, esterase
  

Answer 1

The correct option is B Restriction enzymes, polymerase enzymes, ligases

Restriction enzymes, polymerase enzymes, ligases, vectors
and the host organism are the tools of recombinant DNA technology. Among these, restriction enzymes, polymerase enzymes and ligases are the main enzymes.

Restriction enzymes are used to cut the DNA at specific sites.

Polymerase enzymes are used to synthesise nucleic acids during amplification.

Ligase is used to join the DNA fragments cut by the restriction enzymes.