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Question

Which of the following is commonly used as vector for introducing a DNA fragment in human lymphocytes?

A
λphage
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B
Ti plasmid
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C
Retrovirus
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D
pBR 322
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Solution

The correct option is C Retrovirus
Retroviruses are the only animal viruses that are known to require the stable integration of their genetic information into the genomes of the host cells they infect. Hence it is commonly used as vector for introducing a DNA fragment in human lymphocytes.
Phage λ (lambda phage) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli. The recombinant lambda DNA-containing inserts is packaged into infectious phage particles using an efficient in vitro packaging reaction. Once the particles are formed, each one can inject its DNA into an E. coli cell.
The Ti plasmid can be used to carry foreign genes into plant cells.
pBR322 DNA is a commonly used plasmid cloning vector in E. coli.

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