Question

Which of the following is correct match of phenomenon and its explanation?

• A. Central dogma$\to$ RNA$\to$ DNA $\to$ Protein$\to$ RNA
• B. Reverse transcription$\to$ PCR$\to$ DNA amplification
• C. Transcription$\to$ Formation of RNA and proteins
• D. RNA silencing$\to$ Use of dsRNA

Answer 1

The correct option is B Reverse transcription$\to$ PCR$\to$ DNA amplification

Reverse transcription polymerase chain reaction (RT-PCR) uses mRNA rather than DNA as the starting template. First, the enzyme reverse transcriptase uses the mRNA template to produce a complementary single-stranded DNA strand called cDNA in a process known as reverse transcription. Next, DNA polymerase is used to convert the single-stranded cDNA into double-stranded DNA. These DNA molecules can now be used as templates for a polymerase chain reaction ( PCR ) for DNA amplification.

Central dogma can be correctly represented as DNA→ DNA→ RNA → Protein.

Transcription is the formation of RNA from DNA.

So, the correct option is 'Reverse transcription→ PCR→ DNA amplification'.