The correct option is B Reproductive isolation
Speciation is the formation of new and distinct species in the course of evolution.
Small and random genetic changes or mutations are created within a population's gene pool all the time. These changes accumulate over long periods in a population, to a point that sexual isolation from the rest of the population happens, and this leads to speciation.
Seasonal isolation - any variation in seasons that effectively keeps two populations of organisms from interbreeding.
Behavioural isolation - is an important evolutionary mechanism that helps members of the same species identify each other as proper mates.
Tropical isolation is not a term commonly used in evolution.