Question

Which of the following is not a desirable feature of a cloning vector?

Answer 1

Cloning vectors are small genome, where foreign DNA can be inserted and delivered to the host cell that can be used for cloning purposes. Example- plasmid, bacteriophage, cosmids.

A cloning vector should possess an origin of replication so that it can self-replicate inside the host cell.
It should have a restriction site for the insertion of the target DNA.
It should have a selectable marker with an antibiotic resistance gene that facilitates screening of the recombinant organism.

Presence of more than one recognition site within vector will create multiple fragments which will complicate gene cloning.