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Question

Which of the following is not a tool in genetic engineering?

A
Restriction endonucleases and ligases
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B
Exonuclease
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C
Reverse transcriptase
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D
Vectors and Markers
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Solution

The correct option is B Exonuclease
Genetic engineering procedures require various molecular tools such as restriction endonucleases, ligases, reverse transcriptases, vectors and markers. Restriction exonucleases are enzymes that cleave nucleotides from the end of a polynucleotide chain. These enzymes do not have a specific cut site or recognition sequence, hence are not used in normal genetic engineering procedures as they require high specificity.

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