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Question

Which of the following restriction enzyme/endonuclease is most widely used in genetic engineering?

A
RE Type I
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B
RE Type II
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C
RE Type III
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D
Both A & C
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Solution

The correct option is B RE Type II
  • There are three classes of restriction enzymes, labeled type I, II, and III.
  • Type I restriction systems have a single enzyme that modifies (methylation) the DNA strand at a specific recognition site but restriction activities occur randomly and away from the recognition site.
  • Type III restriction systems have separate enzymes for restriction activity and methylation and cleave outside the restriction site.
  • Thus, both Type I and III are not used in genetic engineering.
  • While Type II restriction enzymes are highly useful in recombinant DNA techniques because they have a separate specific enzyme for restriction which cleaves at the specific sequence site.
  • One enzyme recognizes and cuts the DNA, the other enzyme recognizes and methylates the DNA.

Thus, the correct answer is option B.

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