Which of the following is not an enzyme involved in DNA replication?
RNA primer is a short segment of nucleotides (about 5-10 nucleotides in length) which are the first few bases added by the enzyme RNA primase at the beginning of DNA replication. RNA primers are necessary because the main enzyme that adds nucleotides, DNA polymerase, can only add nucleotides to an already existing strand of nucleotides. Thus, the RNA primer basically serves to prime and lay a foundation for DNA synthesis. DNA polymerase then takes over by adding nucleotides, beginning with the 3’ end of the RNA primer (in the 5’→ 3’ direction).
Helicase is the enzyme that unwinds the double helical DNA by breaking hydrogen bonds between the two strands. For a DNA to replicate, the two strands of the double helix have to separate.
In DNA replication, ligase links the Okazaki fragments together to make one continuous strand of DNA.