The correct option is D This is a sequence of DNA in a genome which inhibits DNA replication.
The origin of replication "ori" is asequence where replication starts. This sequence is also responsible for controlling the vector copy number which could typically be in the range of 25–50 copies/cell if the expression vector is derived from the low-copy-number plasmid pBR322, or between 150 and 200 copies/cell if derived from the high-copy-number plasmid pUC.