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Question

Which of the following is not true for ori ?

A
This is a sequence from where replication starts
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B
Any piece of DNA when linked to this sequence can be made to replicate within the host cells
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C
This sequence is also responsible for controlling the vector copy number
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D
This is a sequence of DNA in a genome which inhibits DNA replication.
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Solution

The correct option is D This is a sequence of DNA in a genome which inhibits DNA replication.
The origin of replication "ori" is asequence where replication starts. This sequence is also responsible for controlling the vector copy number which could typically be in the range of 25–50 copies/cell if the expression vector is derived from the low-copy-number plasmid pBR322, or between 150 and 200 copies/cell if derived from the high-copy-number plasmid pUC.

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