Question

Which of the following is not true w.r.t. copy number of the linked DNA in the vector?

• A. It is equal to the copy number of vector in host cell
• B. It is controlled by ori sequence
• C. It is controlled by selectable marker
• D. Depends on the sequence from where replication starts

Answer 1

The correct option is C It is controlled by selectable marker

ori sequence controls the copy number of the linked DNA in the vector.
Origin of replication (ori) is a sequence from where replication starts. If one wants to recover many copies of the target DNA it should be cloned in a vector whose origin supports high copy number.