Question

Which of the following is required to perform polymerase chain reaction?

• A. Primers, dNTPs and DNA polymerase
• B. DNA, Ca$C{l}_2$ and nuclease
• C. $M{g}^{+2}$, DNABoth (a) and (c)
• D. Both (a) and (c)

Answer 1

The correct option is D Both (a) and (c)

According to the components and relevant steps of Polymerase chain reaction, primers, dNTPs and DNA polymerase are used for DNA synthesis.

DNA is used as a template and Mg$+$ is necessary for DNA synthesis.

So the correct option is 'Both (a) and (c)'.