Question

Which of the following statements are correct for the enzyme Taq polymerase?

(i) It remains active during the high temperature-induced denaturation of dsDNA.

(ii) It requires primers for carrying out the process of polymerisation.

(iii) It synthesizes the RNA region between the primers, using $dNTPs$ and $M{g}^{2+}$ _______________.

• A. (i) and (ii)
• B. (ii) and (iii)
• C. (i), (ii) and (iii)
• D. None of these

Answer 1

The correct option is A (i) and (ii)

The final step of PCR is the extension, wherein Taq DNA polymerase (isolated from a thermophilic bacterium Thermus aquaticus) synthesizes the DNA region between the primers, using dNTPs (deoxynucleoside triphosphates) and $M{g}^{2+}$. The primers are extended towards each other so that the DNA segment lying between the two primers is copied. The optimum temperature for this polymerisation step is ${72}^o$$C$. Taq polymerase remains active during high temperature-induced denaturation of double-stranded DNA.