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Question

Which of the following is used for observing spindle fibres?

A
Dark field microscope
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B
Phase contrast microscope
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C
Polarisation microscope
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D
Scanning transmission electron microscope
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Solution

The correct option is B Phase contrast microscope
Various contrast microscopes enhance the contrast and, hence, are able to reveal many cellular features without elaborate staining procedures which cannot be revealed by traditional microscopy. Contrast microscopes have been widely used to study cell divisions. Spindle formation can be studied using contrast microscopes. Scanning electron microscopy will reveal surface topology. In dark field microscopy, the field is dark and the sample is illuminated. It is a variant of conventional light microscopy. In polarisation microscope, polarised light is used to enhanced visibility.
So, the correct answer is 'Phase contrast microscope'

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