Question

Which of the following is used to fuse adjacent plasma during hybridoma technology?

• A. Polyethylene glycol
• B. Ploy butylene glycol
• C. Magnesium chloride
• D. Calcium chloride

Answer 1

The correct option is A Polyethylene glycol

The process by which protoplasts of two cells are fused to form a hybrid is known as hybridization. In hybridoma technology, the protoplasts of the B-cells and the myeloma cells are fused using the agent polyethylene glycol. This agent along with calcium ions and sodium ions causes the development of charges on the surface of the cell membrane. This results in the packaging of the protoplasts in the cell membrane due to the activities of the ions with the cytoplasmic proteins. The cell which is produced by this method is used for the production of monoclonal antibodies.

Thus, the correct answer is option A.