Question

Which of the following produces androgenic haploids in anther cultures?

• A. Anther wall
• B. Tapetal layer of anther wall
• C. Connective tissue
• D. Young pollen grains

Answer 1

Answer: (D)

Young pollen grains

Pollen culture is a technique of production of haploid plants by culture of pollen grains obtained from anther of a plant under aseptic condition on artificial media of known composition. The technique was discovered by Guha and Maheshwari. The pollen's were obtained by squeezing the anthers after surface sterilizing with hypochlorite. The obtained pollen's were cultured on nutrient media.

The cells of connective tissue, tapetal layer of anther wall and anther wall are diploid in nature so they produce diploid plant.

So, the correct answer is option D.