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Question
Which of the following restriction enzymes produces blunt ends?
Which of the following restriction enzymes produces blunt ends?
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Solution
The correct option is C Eco RV
Restriction enzymes cleave the phosphodiester bonds present between consecutive nucleotides within or near to specific DNA sequences known as recognition sequences.
Blunt ends are produced when restriction enzymes cut both the strands of double stranded DNA in a straight manner at the same position.
Sticky ends are the single stranded, unpaired bases (overhangs) produced in the DNA fragments. It happens when the restriction enzyme cleaves at a point slightly away from the centre of the palindromic sequence, however between the same two bases.
More than 900 restriction enzymes have been isolated from over 230 strains of bacteria, each of which recognise different recognition sequences.
Eco RV, Hind III, Sal I and Xho I are the restriction enzymes obtained from certain strains of different bacteria.
Below is a table showing the type of DNA fragments produced by them:
Restriction enzymes cleave the phosphodiester bonds present between consecutive nucleotides within or near to specific DNA sequences known as recognition sequences.
Blunt ends are produced when restriction enzymes cut both the strands of double stranded DNA in a straight manner at the same position.
Sticky ends are the single stranded, unpaired bases (overhangs) produced in the DNA fragments. It happens when the restriction enzyme cleaves at a point slightly away from the centre of the palindromic sequence, however between the same two bases.
More than 900 restriction enzymes have been isolated from over 230 strains of bacteria, each of which recognise different recognition sequences.
Eco RV, Hind III, Sal I and Xho I are the restriction enzymes obtained from certain strains of different bacteria.
Below is a table showing the type of DNA fragments produced by them:
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