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Question
Which of the following statements does not hold true for restriction enzymes?
Which of the following statements does not hold true for restriction enzymes?
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Solution
The correct option is B It is isolated from viruses
It recognises a palindromic nucleotide sequence:
Palindromic sequences are the sequences that read the same in both the DNA strands when read from the 5′→3′ direction.
Restriction enzymes are a type of endonucleases that cleave the phosphodiester bonds present between consecutive nucleotides at or near to specific positions called ‘recognition sites’. The cleavage site is present within the DNA. The cut is made by breaking the phosphodiester bonds between specific nucleotides of the palindromic sequence on both the strands of the DNA.
It is an endonuclease:
Restriction enzymes are endonucleases as they make cuts within the DNA strand and not at the outer ends.
It is isolated from viruses:
Restriction enzymes naturally exist in bacteria and are used as defense mechanisms against bacteriophage. More than 900 restriction enzymes which have unique recognition sites have been isolated from more than 230 strains of bacteria.
Viruses do not possess restriction enzymes.
It can produce the same kind of sticky ends in different DNA molecules:
When cut with the same restriction enzyme, different DNA molecules such as the DNA of interest and the vector DNA, produce the same complementary sticky ends.
It recognises a palindromic nucleotide sequence:
Palindromic sequences are the sequences that read the same in both the DNA strands when read from the 5′→3′ direction.
Restriction enzymes are a type of endonucleases that cleave the phosphodiester bonds present between consecutive nucleotides at or near to specific positions called ‘recognition sites’. The cleavage site is present within the DNA. The cut is made by breaking the phosphodiester bonds between specific nucleotides of the palindromic sequence on both the strands of the DNA.
It is an endonuclease:
Restriction enzymes are endonucleases as they make cuts within the DNA strand and not at the outer ends.
It is isolated from viruses:
Restriction enzymes naturally exist in bacteria and are used as defense mechanisms against bacteriophage. More than 900 restriction enzymes which have unique recognition sites have been isolated from more than 230 strains of bacteria.
Viruses do not possess restriction enzymes.
It can produce the same kind of sticky ends in different DNA molecules:
When cut with the same restriction enzyme, different DNA molecules such as the DNA of interest and the vector DNA, produce the same complementary sticky ends.
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