Question

Which of the following statements is/are correct about heat shock.

• A. A step of the process that enables introduction of alien DNA into the host cells
• B. Both A and C
• C. A step of the process that enables linking of alien DNA to the plasmid
• D. Given at 42°C

Answer 1

The correct option is B Both A and C

Process of heat shock method

Process of heat shock method is as follows:

• Bacterial cells are treated with divalent ions (Ca2+).
• Then a heat shock at 42℃ is provided for a brief time period which creates transient pores within the bacterial cell membrane.
• These pores allow the DNA of interest to enter into the bacteria.
• These cells are placed back on ice to retain the plasmids inside the bacteria.

Final answer

(d) Both A and C