1
Question
Which of the following statements is/are correct?
I. Recombinant DNA technology requires both nucleases and ligases.
II. Ligases produce nicks in the recombinant DNA molecule.
III. Nucleases produce the nick in the DNA molecule.
Which of the following statements is/are correct?
I. Recombinant DNA technology requires both nucleases and ligases.
II. Ligases produce nicks in the recombinant DNA molecule.
III. Nucleases produce the nick in the DNA molecule.
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Solution
The correct option is C I and III
Recombinant DNA (rDNA) is a modified DNA molecule that has genes from multiple biological sources that are not normally associated with each other. The technology used for producing DNA of desired sequence (rDNA) artificially is referred to as recombinant DNA technology.
The rDNA is based on the concept of introducing fragment of DNA having desired gene(s) into the genetic material of another organism and integrating them to generate one molecule. This would help to generate the desired product. To obtain fragments of DNA containing specific sequences, the parent DNA molecule needs to be cleaved. Once the desired fragments of DNA are obtained from different biological sources, they are ligated (joined together). The molecule obtained as a result is called recombinant DNA.
Thus, in order to make rDNA molecule, the ideal requirements would include the enzymes that can cut the DNA at desired sites and enzymes that can ligate the desired fragments of DNA.
Nucleases are the class of enzymes that digest the nucleic acids. They are of two types - endonucleases and exonucleases. Preparation of rDNA requires a class of endonuclease called restriction endonucleases. These enzymes cleave the phosphodiester bonds present between the nucleotides within the DNA molecule at or near to specific sequences that they identify (called recognition sites). This cleaving of DNA is referred to as nicking. Therefore, nucleases are required for nicking the DNA in genetic engineering.
The desired fragments of DNA are required to be ligated. This is done with the help of DNA ligase. DNA ligase help in formation of phosphodiester bonds between those fragments of DNA taken for use.
Hence, DNA ligase and nucleases are required in genetic engineering. Therefore, statements I and III are correct.
Recombinant DNA (rDNA) is a modified DNA molecule that has genes from multiple biological sources that are not normally associated with each other. The technology used for producing DNA of desired sequence (rDNA) artificially is referred to as recombinant DNA technology.
The rDNA is based on the concept of introducing fragment of DNA having desired gene(s) into the genetic material of another organism and integrating them to generate one molecule. This would help to generate the desired product. To obtain fragments of DNA containing specific sequences, the parent DNA molecule needs to be cleaved. Once the desired fragments of DNA are obtained from different biological sources, they are ligated (joined together). The molecule obtained as a result is called recombinant DNA.
Thus, in order to make rDNA molecule, the ideal requirements would include the enzymes that can cut the DNA at desired sites and enzymes that can ligate the desired fragments of DNA.
Nucleases are the class of enzymes that digest the nucleic acids. They are of two types - endonucleases and exonucleases. Preparation of rDNA requires a class of endonuclease called restriction endonucleases. These enzymes cleave the phosphodiester bonds present between the nucleotides within the DNA molecule at or near to specific sequences that they identify (called recognition sites). This cleaving of DNA is referred to as nicking. Therefore, nucleases are required for nicking the DNA in genetic engineering.
The desired fragments of DNA are required to be ligated. This is done with the help of DNA ligase. DNA ligase help in formation of phosphodiester bonds between those fragments of DNA taken for use.
Hence, DNA ligase and nucleases are required in genetic engineering. Therefore, statements I and III are correct.
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