Question

Which of the following statements is correct regarding phase microscopy?

• A. Used for the study of living cells.
• B. Based on the scattering of light and uses a dark field condenser.
• C. Related to retardation and to the thickness of the object.
• D. The best method for studying non living ultrastructures.

Answer 1

The correct option is A Used for the study of living cells.

Before the introduction of the phase contrast microscope, it was difficult to observe living cells. As living cells are translucent, they must be stained to tbe visible in a traditional light microscope. Unfortunately, the process of staining cells generally kill the cells. With the invention of the phase contrast microscopy, it became possible to observe unstained living cells in details.