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Question
Which of the following tools are used in recombinant DNA technology?
Which of the following tools are used in recombinant DNA technology?
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Solution
The correct option is D All of these
Recombinant DNA (rDNA) refers to DNA that has been formed artificially by combining DNA segments from one or more organisms into another host DNA by using various tools.
Recombinant DNA technology (rDNA technology) is a technique that alters the phenotype of an organism when rDNA is introduced and incorporated into the genome of the host.
The various tools used in the creation of recombinant DNA are:
1. Restriction enzymes: These enzymes are used to cut the DNA molecule at specific sequences. These are also called molecular scissors.
2. DNA ligase: It helps in the linking of DNA molecules. It is also called molecular glue.
3. Vector: A DNA molecule that is used as a vehicle to carry the desired gene (gene of interest) into the host.
The most commonly used vectors are plasmids. Plasmids are extrachromosomal circular rings of DNA found in certain bacterial cells. They are self-replicating and hence when introduced into the host organism/cell, they are able to replicate independently and thus the gene of interest also replicates.
Procedure of rDNA technology: It involves identifying the gene of interest in the desired organism. The restriction enzymes cut the DNA at specific sites and help in isolation of the desired gene. Restriction enzymes are also used to cut the vector DNA. The desired gene is then linked to the vector molecule. DNA ligase helps in linking the gene of interest to the vector DNA. Thus, rDNA is formed.
Recombinant DNA (rDNA) refers to DNA that has been formed artificially by combining DNA segments from one or more organisms into another host DNA by using various tools.
Recombinant DNA technology (rDNA technology) is a technique that alters the phenotype of an organism when rDNA is introduced and incorporated into the genome of the host.
The various tools used in the creation of recombinant DNA are:
1. Restriction enzymes: These enzymes are used to cut the DNA molecule at specific sequences. These are also called molecular scissors.
2. DNA ligase: It helps in the linking of DNA molecules. It is also called molecular glue.
3. Vector: A DNA molecule that is used as a vehicle to carry the desired gene (gene of interest) into the host.
The most commonly used vectors are plasmids. Plasmids are extrachromosomal circular rings of DNA found in certain bacterial cells. They are self-replicating and hence when introduced into the host organism/cell, they are able to replicate independently and thus the gene of interest also replicates.
Procedure of rDNA technology: It involves identifying the gene of interest in the desired organism. The restriction enzymes cut the DNA at specific sites and help in isolation of the desired gene. Restriction enzymes are also used to cut the vector DNA. The desired gene is then linked to the vector molecule. DNA ligase helps in linking the gene of interest to the vector DNA. Thus, rDNA is formed.
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