The correct option is D All of the above
A vital stain in a casual usage may mean a stain that can be applied on living cells without killing them. Vital stains have been useful for diagnostic and surgical techniques in a variety of medical specialties. In supravital staining living cells that have been removed from an organism, whereas intravital staining is done by injecting or otherwise introducing the stain into the body. Janus green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally. Methylene blue is a supravital stain. Neutral red can be used as a vital stain. Live cells incorporate neutral red into their lysosomes.