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Question

Which one of the following graphs shows the relationship between the rate of an enzymatic activity and substrate concentration(S)?


A

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B

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C

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D

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Solution

The correct option is D


Increase in substrate concentration increases the rate of reaction due to two factors: (i) occupation of more and more active sites by the substrate molecules: (ii) higher number of collisions between substrate molecules.

The rise in velocity is quite high in the beginning but it decreases progressively with the increase in substrate concentration. If a graph is plotted for substrate concentration versus reaction velocity, it appears as a hyperbolic curve. A stage is reached where velocity is maximum. It does not increase further by increasing the substrate concentration. At this stage, the enzyme molecules become fully saturated and no active site is left free to bind additional substrate molecule.


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