Question

Which one of the following is the correct sequence of procedures for site specific somatic hybridization in plants?

• A. Procuring haploid cells from the parental species-isolation of protoplasts by removing the cellulose wall-protoplast fusion-regeneration of the fused cell
• B. Isolation of somatic cells-extraction of protoplasts-protoplast fusion - culturing of the fused cell
• C. Isolation of haploid and somatic cells extraction of their protoplasts regeneration-fusion of cells
• D. Enzymatic isolation of haploid cells- isolation of protoplasts fusion of extracted protoplasts-regeneration of the fused cell

Answer 1

The correct option is B Isolation of somatic cells-extraction of protoplasts-protoplast fusion - culturing of the fused cell

Several steps are involved in the somatic hybridization process such as-

(1) the source of protoplasts

(2) the isolation of protoplasts

(3) plating of protoplasts

(4) regeneration of plants

(5) fusion of protoplasts

(6) selection procedures

(7) identification and characterization of the somatic hybrid plants.

So, the correct option is B.