Which one of the following is used as vector for cloning genes into higher organisms?
A
Salmonella typhimurium
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B
Rhizopus-nigricans
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C
Retrovirus
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D
Baculovirus
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Solution
The correct option is A Retrovirus
Retroviruses are RNA containing viruses that replicateitself by first reverse-coding its genes into the DNA of the cells it infects. Once transformed from RNA to DNA, the viral DNA can be integrated into the genome of the infected cells.
They areinanimals have the ability to transform normal cells into cancerous cells. Scientists have transformed these pathogens into useful vectors for delivering genes of interest to humans.
Retroviruses have been disarmed and are now used to deliver desirable genes into animal cells. So, once a gene or a DNA fragment has been ligated into a suitable retroviral vector it is transferred into a bacterial, plant or animal host (where it multiplies).