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Question

Which one of the following tests used for the identification of functional groups in organic compounds does not use copper reagent?

A
Seliwanoff's test
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B
Barfoed's test
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C
Biuret test for peptide bond
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D
Benedict's test
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Solution

The correct option is A Seliwanoff's test
Seliwanoff's test is used to distinguish aldoses and ketoses. Reagent used is resorcinol dissolved in conc HCl.

Barfoed’s Test:
It is given by reducing monosaccharides. Reagent used is Cupric acetate and acetic acid.

Biuret test is used to check the presence of the peptide bond.
The Biurette reagent made of sodium hydroxide and copper (II) sulphate.

Benedict's test:
It is used to detect the presence of reducing sugars in sample.
Its reagent is made up of a complex mixture of sodium citrate, sodium carbonate, and the pentahydrate of copper(II) sulphate.

Thus, option (a) is the correct answer.

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