Question

Which one of the incorrect statement regarding DNA polymerase III?

• A. It is used to ligate any DNA fragment
• B. Can directly bind to ori site
• C. It is isolated from a virus
• D. All of (A), (B) and (C)

Answer 1

Answer: (D)

All of (A), (B) and (C)

A. Ligation refers to the joining of two DNA fragments through the formation of a phosphodiester bond. An enzyme known as ligase catalyzes the ligation reaction.

B. During initiation, proteins bind to the origin of replication while helicase unwinds the DNA helix and two replication forks are formed at the origin of replication.

C. It is not isolated from a virus.

DNA polymerase III is essential for the replication of the leading and lagging strands.

So, all statements are incorrect regarding DNA polymerase III.