Question

Which technique can be used to study the means of binding a specific protein to a regulatory DNA sequence?

Answer 1

Binding a specific protein to a regulatory DNA sequence

1. Proteins recognize a particular sequence by having a surface that is complementary to that of DNA.
2. It forms a series of electrostatic and Van der Waals interactions.

X-rays crystallography

1. X-ray crystallography can be used to study the binding of a specific protein.
2. This technique was used by Crick, Wilkins, and Watson to find the molecular configuration of the double helix structure of DNA.
3. In order to ascertain the molecular and atomic structure of a crystal, X-ray crystallography uses electromagnetic radiation, more specifically, X-rays.
4. This technique is based on X-ray diffraction.
5. An X-ray beam may be diffracted when it hits a crystal.
6. A three-dimensional representation of the density of electrons within the crystal can be created using the angles and intensities of these diffracted beams.
7. It is possible to determine the location and arrangement of electrons within the crystalline structure by analysing the intensities and angles of these beams.
8. With this technique, we can know about the configuration of atoms and structures of proteins, enzymes, and DNA.