Question

Which vector can clone only a small fragment of DNA?

Answer 1

Plasmid:

1. Plasmids can clone only a small fragment of DNA.
2. They are small, circular, extrachromosomal DNA which is present in bacteria and can replicate autonomously.
3. Plasmids are extra chromosomal, autonomously replicating circular, double-stranded DNA molecules.
4. They naturally occur in some yeasts and several bacteria.
5. These plasmids are altered to be used as vectors as they can clone DNA fragments close to size 10kbp.
6. Plasmid pBR322 is one of the earliest and most common cloning vectors.