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Question
Which vector is commonly used in the transfer of gene in a crop plant?
Which vector is commonly used in the transfer of gene in a crop plant?
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Solution
The correct option is C Ti-plasmids of Agrobacterium
- Plasmids are low molecular weight extrachromosomal DNA that carries the origin of replication, restriction sites, and selectable markers to confer readily selectable phenotypic traits on host cells.
- Plasmid vector from B subtilis, pBP26, can clone the DNA segment of 10-15 kb.
- Bacteriophages have linear DNA that is cut to insert the gene of interest thereby generating chimeric DNA and used as a vector but has limitation on the size of cloned DNA as too large DNA segment cannot be accommodated into a small phage head.
- Agrobacterium tumefaciens have the capacity to transfer its Ti and Ri plasmids to host cells, which allow them to serve as vectors for large DNA segments up to 25 kb. The transformed cell is easily identified by the presence of tumors.
- The E coli phage contains an origin of replication, few essential genes, single-stranded protruding cohesive ends of 12 bases which allow it to anneal to form cos sites where a gene of interest is cloned. Size of eukaryotic genes are big enough which cannot be cloned into plasmids or phage vectors.
So, the correct answer is option C.
- Plasmids are low molecular weight extrachromosomal DNA that carries the origin of replication, restriction sites, and selectable markers to confer readily selectable phenotypic traits on host cells.
- Plasmid vector from B subtilis, pBP26, can clone the DNA segment of 10-15 kb.
- Bacteriophages have linear DNA that is cut to insert the gene of interest thereby generating chimeric DNA and used as a vector but has limitation on the size of cloned DNA as too large DNA segment cannot be accommodated into a small phage head.
- Agrobacterium tumefaciens have the capacity to transfer its Ti and Ri plasmids to host cells, which allow them to serve as vectors for large DNA segments up to 25 kb. The transformed cell is easily identified by the presence of tumors.
- The E coli phage contains an origin of replication, few essential genes, single-stranded protruding cohesive ends of 12 bases which allow it to anneal to form cos sites where a gene of interest is cloned. Size of eukaryotic genes are big enough which cannot be cloned into plasmids or phage vectors.
So, the correct answer is option C.
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