Question

While sequencing the DNA fragment in a BAC, it is further cut into fragments containing 2000 base pairs. How are the DNA fragments from BACs separated from the human DNA?

• A. BAC DNA is cut out early and only human DNA is cut into smaller fragments
• B. The DNA sequence of BAC is known, therefore during tallying of DNA sequence of all fragments, the BAC DNA is left out
• C. BAC DNA has specific markers that can be read and identified while sequencing
• D. None of the above

Answer 1

The correct option is B The DNA sequence of BAC is known, therefore during tallying of DNA sequence of all fragments, the BAC DNA is left out

The first step before DNA Sequencing commences is the cutting of human DNA along with the BAC DNA into smaller fragments. All the fragments are then sequenced and superimposed on the already existing sequence of the BAC. This way the human DNA sequence is obtained and stored.