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Question

Who performed the popular "Replica Plating Experiment" :-

A
Nirenberg and Kornberg
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B
Kornberg and Lederberg
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C
Joshua Lederberg and Esther Lederberg
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D
Holley, Khorana and Mathai
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Solution

The correct option is C Joshua Lederberg and Esther Lederberg
Replica plating was first described by Esther Lederberg and Joshua Lederberg in 1952.

Microbiological technique in which one or more secondary Petri plates containing different solid (agar-based) selective growth media (lacking nutrients or containing chemical growth inhibitors such as antibiotics) are inoculated with the same colonies of micro-organisms from a primary plate (or master dish), reproducing the original spatial pattern of colonies. The technique involves pressing a velveteen-covered disk, and then imprinting secondary plates with cells in colonies removed from the original plate by the material. Generally, large numbers of colonies (roughly 30300) are replica plated due to the difficulty in streaking each out individually onto a separate plate.

The correct answer is option C.

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