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Question

Why is isopropanol used in DNA extraction?


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Solution

Isopropanol in DNA extraction:

  1. Isopropyl alcohol is a colourless, flammable, and pungent chemical substance.
  2. The purpose of salt and ethanol/isopropanol is to precipitate DNA from a solution.
  3. The salts neutralize the negatively charged phosphate in DNA's negative charge, while the isopropanol/ethanol removes the phosphate's H2O hydration shell.

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